Journal: PLOS One

Article Title: A novel peptide mimetic, brilacidin, for combating multidrug-resistant Neisseria gonorrhoeae

doi: 10.1371/journal.pone.0325722

Figure Lengend Snippet: A) Propidium iodide fluorescence after treating N. gonorrhoeae with either azithromycin or brilacidin to predict the permeabilization of the cytoplasmic membrane. B) Percentage of ATP leakage from N. gonorrhoeae treated with brilacidin or azithromycin relative to Triton X-100 (positive control with complete ATP leakage). Asterisks denote statistically significant differences between test agents and DMSO (untreated), * (P < 0.05), ** (P < 0.01), and **** (P < 0.0001) as determined by one-way ANOVA.

Article Snippet: Media and reagents including McCoy’s 5A medium and hematin (Sigma Aldrich, St. Louis, MO, USA), triton X-100 (Acros Organics, Fair Lawn, NJ, USA), BacTiter-Glo reagent (Promega Corporation, Madison, WI, USA), Propidium iodide (PI) and nicotinamide adenine dinucleotide (NAD) (Chem-Impex International, Wood Dale, IL, USA), MTS (3-(4,5-dimethylthia- zol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium) (Abcam, Waltham, MA, USA), and brucella broth, chocolate II agar plates, IsoVitaleX and bovine hemoglobin (Becton, Dickinson and Company, Cockeysville, MD, USA), were obtained from chemical vendors.

Techniques: Fluorescence, Membrane, Positive Control