Journal: Antioxidants

Article Title: Hyperbaric Oxygen Improves Cognitive Impairment Induced by Hypoxia via Upregulating the Expression of Oleic Acid and MBOAT2 of Mice

doi: 10.3390/antiox13111320

Figure Lengend Snippet: ( A ) Experimental timeline describing the time of exposing to CON, Hypoxia or HBO in all three groups (n = 10). ( B ) The weight of mice (n = 10). ( C ) Y maze spontaneous alternation. ( D ) The total number of arm entries of the Y maze (n = 6). ( E ) The escape latency. ( F ) The swimming trajectories. ( G ) The number of crossings over the original platform location of CON, Hypoxia, and HBO mice during the test period. The percentages of swimming ( H ) distance and ( I ) time in goal area. ( J ) The speed of exploration of CON, Hypoxia, and HBO mice during the test period (n = 7). ( K ) Western blots of PSD93 protein and corresponding quantitative results in the prefrontal cortex (n = 3). ( L ) Representative images of Nissl body staining (blue spots, 200×) and corresponding quantitative results in the prefrontal cortex (n = 3). Scale bar, 50 μm. ( M ) Representative images of NeuN staining (vivid green dots, 200×) and corresponding quantitative results in the prefrontal cortex (n = 3). The nucleus was stained with DAPI (blue color). Scale bar, 50 μm. Data are presented as mean ± standard error of the mean (SEM). The behavioral changes over time during the training period were analyzed by repeated measure ANOVA. Significant level: ns: not statistically significant, * p < 0.05; ** p < 0.01; *** p < 0.001.

Article Snippet: The brain sections were soaked in Nissl staining solution (Beyotime Biotechnology, Nanjing, China) and dehydrated in 95% ethanol, dimethylbenzene.

Techniques: Western Blot, Staining